The Ancient Magus' Bride does not feature any identifiable LGBTQ+ characters or themes. The narrative primarily focuses on its central heterosexual relationship and fantasy elements, resulting in no direct portrayal of LGBTQ+ identities or experiences.

The Ancient Magus' Bride, 2017, does not include any identifiable transsexual characters or themes in its storyline. The narrative focuses on fantasy elements, magic, and the relationship between a human girl and an ancient sorcerer, without delving into transgender identity or related issues. There are no plot points or character arcs that depict or explore transsexual experiences.

The series primarily features magical combat and interactions with fae creatures. The main female character, Chise Hatori, is not depicted as a physical combatant and relies on her unique magical abilities or the protection of others. No female characters are shown to defeat male opponents in direct physical combat using skill, strength, or melee weapons.

The 2017 anime adaptation of "The Ancient Magus' Bride" faithfully portrays the established genders of its characters as depicted in the original manga series. No significant characters were changed in gender from their source material.

The Ancient Magus' Bride is a Japanese anime adaptation of a Japanese manga. The characters' portrayals in the anime align with their original designs and the cultural context of the source material, without any instances of a character established as one race being depicted as another.