The Flash TV show features openly gay and transgender characters, such as Captain David Singh and Nia Nal (Dreamer). Their portrayals are consistently positive, depicting them with dignity and agency, and normalizing their identities within the superhero narrative. The show avoids harmful stereotypes, contributing to an overall affirming representation of LGBTQ+ individuals.

The Flash features Nia Nal (Dreamer), a transgender woman and superhero. Her portrayal is affirming, depicting her with dignity, agency, and as a powerful, respected member of the superhero community. Her trans identity is handled respectfully, contributing to a positive representation.

The show features Rosalind Dillon as The Top, a character who was canonically and widely established as male (Roscoe Dillon) in the DC Comics source material, thus constituting a gender swap.

The series features Iris West and Wally West, characters who were canonically established as white in the original DC Comics, portrayed by Black actors. These portrayals constitute clear instances of race swaps.