The Flight Attendant features multiple LGBTQ+ characters, including Davey, Shane, and Eli, whose identities are depicted with dignity and normalcy. Their relationships are healthy, and their queer identities are not sources of conflict or negative stereotypes. The portrayal is consistently affirming and integrated into the narrative.

The show adapts a novel where characters Megan Briscoe and Shane Evans were depicted as white. In the series, these roles are portrayed by Latina and Black actors, respectively, constituting race swaps.

The show portrays Judaism primarily through Annie Mouradian's family. While there are comedic elements regarding parental expectations, the depiction is generally respectful, highlighting cultural identity and family bonds. The narrative frames their Jewish background as an integral and positive aspect of their loving, albeit sometimes challenging, family dynamic.