The Nevers features multiple LGBTQ+ characters, including an explicitly lesbian relationship and implied bisexual/pansexual and gay individuals. These identities are integrated naturally and portrayed with dignity and complexity. While characters face adversity, their queer identity is not the source of their suffering or depicted negatively, resulting in an overall affirming portrayal.

The Nevers features Annie Cousens, a trans woman and wife of Dr. Horatio Cousens. Her identity is revealed in a flashback, met with unconditional love and acceptance by Horatio. The show portrays Annie with dignity, affirming her worth and their relationship without resorting to harmful stereotypes or negative framing. The overall impact is positive.

The show portrays Christian institutions and some adherents in Victorian London as largely oppressive, fearful, and hypocritical, particularly in their response to the 'Touched.' The narrative critiques how religion is used to maintain social order and justify prejudice against those deemed different, without offering significant counterbalancing positive portrayals of the faith itself.