The film does not feature any identifiable transsexual characters or themes. While a male character, Miyabi Hanakōji, frequently cross-dresses and exhibits effeminate traits, the narrative does not explore or present his identity as transsexual. His cross-dressing is primarily a character quirk rather than a depiction of gender identity.

The combat in this series primarily revolves around characters known as Qwasers, who utilize elemental superpowers. While female characters engage in fights against male opponents, their victories are consistently achieved through the application of these supernatural abilities, rather than through conventional physical combat skills, strength, or martial arts.

Based on available information, there are no documented instances of characters from 'The Qwaser of Stigmata' manga being portrayed with a different gender in the anime adaptation. All major characters retain their original canonical genders.

The Qwaser of Stigmata is a Japanese anime series. There is no information to suggest that any character, canonically established as one race in source material, was portrayed as a different race within this adaptation.