The Spanish Princess features prominent LGBTQ+ characters, including Edward Stafford and Lina de Cardonnes, who engage in same-sex relationships. The series portrays these relationships with dignity and affection, framing the challenges and tragic outcomes as consequences of societal and political pressures rather than inherent issues with their identities. The overall depiction is supportive and validating.

Lina de Cardonnes, a historical lady-in-waiting to Catherine of Aragon, is portrayed by a Black actress. Historical context indicates that a lady-in-waiting in the 16th-century Spanish court would have been white, constituting a race swap for a named historical figure.

The series unequivocally portrays traditional family values, emphasizing lifelong heterosexual marriage for dynastic purposes, distinct gender roles, and the paramount importance of producing heirs within a religious framework.

The series explores the deep personal faith of characters like Catherine of Aragon, portraying it as a source of strength and moral guidance. While it depicts political maneuvering and hypocrisy within the Church hierarchy, the narrative consistently affirms the virtues and dignity of Christian faith itself, positioning the audience to appreciate genuine piety.

The series features a Jewish converso character whose secret practice of Judaism is depicted with sympathy and respect. The narrative highlights the severe persecution and discrimination faced by Jews, clearly condemning the bigotry and positioning the audience to empathize with the challenges of maintaining faith in a hostile environment.