Tosh.0, a comedy show, frequently features internet videos and Daniel Tosh's commentary. While not exclusively focused on LGBTQ+ themes, when they appear, they are often subjected to the show's characteristic irreverent and mocking humor. This approach often uses identity as a source of ridicule or relies on stereotypes, resulting in a net problematic portrayal without affirming counterbalances.

Tosh.0 consistently employs a comedic approach that often targets transsexual individuals and themes for ridicule. The show's humor frequently relies on harmful stereotypes and treats trans identity as a punchline, resulting in a net negative portrayal that lacks affirming elements or critical counterbalance.

The show frequently uses Christianity as a target for satirical humor, often mocking its practices, figures, or perceived hypocrisies for comedic effect, without offering counterbalancing positive portrayals.

When Islam is depicted, it is typically through the lens of satire, often referencing extremist groups or cultural stereotypes for comedic effect, consistent with the show's overall critical approach to various belief systems.

Judaism is occasionally featured in satirical segments, often playing on stereotypes or specific cultural practices for comedic purposes, consistently aligning with the show's irreverent and critical tone.