Young Justice offers a significantly positive portrayal of LGBTQ+ characters. Key characters like Aqualad and Halo are depicted with dignity, agency, and their queer identities are integrated respectfully into the narrative, fostering acceptance and validation without being a source of conflict or negative stereotypes.

Young Justice features Halo, a prominent non-binary character who explicitly states their identity and uses they/them pronouns. The series portrays Halo's journey of self-discovery with dignity and complexity. Other characters are consistently accepting and affirming of Halo's identity, without ridicule or negative framing, resulting in a supportive and validating portrayal.

The show features several non-superpowered female characters, such as Batgirl and Artemis, who frequently engage in and win close-quarters physical fights against multiple male opponents using martial arts and combat skills.