An American Tail does not feature any identifiable LGBTQ+ characters or themes. The story is centered on the challenges of immigration and family reunion, with no elements pertaining to queer identity or experiences.

An American Tail does not feature any identifiable transsexual characters or themes. The story centers on a mouse family's immigration experience and their efforts to reunite, with no elements pertaining to gender identity or transition.

The film does not feature any scenes where a female character engages in direct physical combat and defeats one or more male opponents. Female characters are present but are not depicted in such combat roles.

An American Tail is an original animated film, not an adaptation of pre-existing source material with established characters. All characters were created for this film, so there is no prior canon or historical record to establish a different gender for any character.

The film features anthropomorphic mice characters, not human characters with an established race in prior canon or history. Therefore, the concept of a 'race swap' as defined does not apply to this animated movie.