The film does not contain any discernible LGBTQ+ characters or themes. Its storyline is centered on traditional adventure and friendship narratives, with no elements related to queer identity present in the plot or character arcs.

The film "An American Tail: The Treasure of Manhattan Island" does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of Fievel Mousekewitz and his friends, with no elements related to transgender identity.

The film does not depict any scenes where a female character engages in and wins close-quarters physical combat against one or more male opponents. Female characters are present but do not participate in such combat scenarios.

The film features established characters from the 'An American Tail' series, such as Fievel and Tanya, who retain their original genders. New characters introduced do not have prior canonical genders to be swapped from.

The film primarily features anthropomorphic animal characters, for whom the concept of human racial categories does not apply. New human characters introduced do not have prior established racial identities that were altered.