Batman: Gotham Knight does not depict any LGBTQ+ characters or themes. The narrative focuses exclusively on Batman's early career and encounters with various criminals in Gotham City, with no elements related to queer identity present.

Batman: Gotham Knight, an animated anthology film, does not include any transsexual characters or explore related themes. The narrative focuses on Batman's early career and encounters with various criminals, without any depiction of transgender identity.

The film features female characters such as Detective Anna Ramirez and Cassandra, a martial arts instructor. While Cassandra demonstrates advanced combat skills during a training sequence with Bruce Wayne, neither she nor any other female character is depicted defeating one or more male opponents in direct physical combat.

All established characters in "Batman: Gotham Knight" maintain their canonical gender from the source material. The film introduces new characters, but none are gender-swapped versions of pre-existing male or female characters.

This animated anthology film features established DC characters like Batman, Alfred, Commissioner Gordon, Lucius Fox, and Deadshot. All characters are depicted consistently with their long-standing canonical racial identities from the source material, with no instances of a character's race being altered.