Batman: Gotham Knight does not depict any LGBTQ+ characters or themes. The narrative focuses exclusively on Batman's early career and encounters with various criminals in Gotham City, with no elements related to queer identity present.

Batman: Gotham Knight, an animated anthology film, does not include any transsexual characters or explore related themes. The narrative focuses on Batman's early career and encounters with various criminals, without any depiction of transgender identity.

The film features female characters such as Detective Anna Ramirez and martial arts instructor Cassandra. Anna Ramirez participates in a shootout, but her victories are not through close-quarters physical combat. Cassandra demonstrates martial arts techniques during training sessions with Bruce Wayne, but these are not presented as combat victories against male opponents.

All established characters in "Batman: Gotham Knight" maintain their canonical gender from the source material. The film introduces new characters, but none are gender-swapped versions of pre-existing male or female characters.

This animated anthology film features established DC characters like Batman, Alfred, Commissioner Gordon, Lucius Fox, and Deadshot. All characters are depicted consistently with their long-standing canonical racial identities from the source material, with no instances of a character's race being altered.