Cars 2 does not feature any identifiable LGBTQ+ characters, relationships, or themes. The narrative centers on talking cars in a spy and racing plot, with no elements related to queer identity or experience.

Cars 2 does not feature any identifiable transsexual characters or themes. The narrative centers on talking cars involved in a spy adventure and racing, without any elements that could be interpreted as a portrayal of transgender identity. Therefore, no assessment of positive, negative, or neutral impact can be made.

The film features Holley Shiftwell, a female spy car, who participates in action sequences. However, her victories and combat effectiveness are primarily achieved through advanced technological gadgets and high-speed maneuvers, rather than direct physical combat or martial arts against male opponents.

Cars 2 is a direct sequel where all returning characters maintain their established gender from the previous installment. New characters introduced in the film do not qualify as gender swaps under the given definition.

The characters in "Cars 2" are anthropomorphic vehicles, not human characters. The concept of race, as defined for human characters in the key definition, does not apply to cars. Therefore, no race swaps can occur.