The film 'Daniel Boone: The Wilderness Road' does not include any identifiable LGBTQ+ characters or themes. Consequently, there is no portrayal to evaluate regarding positive, negative, or neutral representation of queer identities within its story.

The film "Daniel Boone: The Wilderness Road" (1961) does not feature any identifiable transsexual characters or themes. Its narrative focuses on historical adventure, and there is no depiction of transgender identity within the story.

The film does not depict any female characters engaging in or winning close-quarters physical combat against male opponents. Female roles are primarily supportive and domestic, with no instances of direct physical confrontation.

The film portrays the historical figure Daniel Boone as male, consistent with his documented gender. There is no evidence of other historically or canonically established characters being depicted with a different gender.

Daniel Boone, a real historical figure of documented white ancestry, is portrayed by a white actor in the film. There is no evidence of any other canonically or historically established character being depicted as a different race.