Demon City Shinjuku does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses entirely on supernatural horror and action, with a heterosexual romantic subplot, and contains no elements related to LGBTQ+ representation.

The film "Demon City Shinjuku" does not feature any identifiable transsexual characters or themes. The narrative focuses on supernatural horror and action, with no elements related to gender identity or transgender experiences.

The film features Sayaka Rama, the primary female character, who possesses psychic and spiritual abilities. However, she does not engage in or win any direct physical combat against male opponents using hand-to-hand or melee weapon skills. Her role is primarily supportive and spiritual.

The 1988 anime film "Demon City Shinjuku" is a direct adaptation of the novel series by Hideyuki Kikuchi. All major characters, such as Kyoya Izayoi, Sayaka Rama, and Levih Rah, maintain their established genders from the source material in the film.

Demon City Shinjuku (1988) is a Japanese anime film adapting a Japanese novel. There is no evidence of any character being canonically established as one race in the source material and then portrayed as a different race in this adaptation.