Despicable Me 2 is a family-oriented animated film centered on Gru's transition from villain to agent, and his budding romance. The movie does not feature any identifiable LGBTQ+ characters, relationships, or themes, resulting in an N/A rating for its portrayal.

Despicable Me 2 does not include any identifiable transsexual characters or themes. The narrative focuses on Gru's adventures as a spy and father, with no elements related to transgender identity. Therefore, the film has no net impact on the portrayal of transsexual individuals.

The film features Lucy Wilde as a skilled agent who participates in action sequences. However, her methods primarily involve gadgets and technology. There are no instances where she or any other female character engages in and wins close-quarters physical combat against one or more male opponents.

Despicable Me 2 is a direct sequel that continues the established characters from the first film with their original genders. All new characters introduced in this installment do not have a prior canonical gender to swap from.

Despicable Me 2 introduces new characters, but all returning characters from the original film maintain their established racial depictions. No character who was canonically established as one race is portrayed as a different race.