Garfield: A Tail of Two Kitties does not feature any identifiable LGBTQ+ characters or themes. The film's plot centers on a comedic animal adventure, and therefore, there is no portrayal to evaluate as positive, negative, or neutral.

Garfield: A Tail of Two Kitties is a family-oriented comedy centered on a cat's adventures. The film does not feature any transsexual characters, storylines, or themes, resulting in no portrayal to evaluate within this framework.

The film is a family comedy centered on animal antics rather than physical combat. No female characters, human or animal, are depicted engaging in or winning direct physical fights against male opponents using skill, strength, or martial arts.

The film features established characters like Garfield, Jon, Odie, and Liz, all of whom retain their canonical genders from the comic strip. New characters introduced for the movie do not constitute gender swaps.

The film features animal characters (cats) and human characters (Jon Arbuckle, Liz Wilson) whose on-screen portrayals align with their established races from the original comic strip and previous adaptations. No character canonically established as one race is depicted as a different race.