GRUFF does not explicitly depict LGBTQ+ characters or themes. The narrative centers on universal themes of familial love, communication, and emotional expression, which may implicitly resonate with LGBTQ+ experiences of seeking acceptance. However, the film's plot does not feature direct LGBTQ+ identities or issues, resulting in no identifiable depiction.

Without specific information about 'GRUFF' (2024) and its content, particularly regarding transsexual characters or themes, a definitive evaluation of its portrayal is not possible. The rating is N/A due to a lack of identifiable depiction details.

The film features a female super spy character, Hazel, whose role implies involvement in combat. However, available sources do not provide specific details or descriptions of scenes where she definitively defeats male opponents in close-quarters physical combat.

The film is an original story with no known source material or historical figures. There is no information indicating any character's on-screen gender differs from an established canonical or historical gender.

GRUFF is an original animated film that establishes its characters as part of a Hispanic family and community. There is no prior source material or established canon from which a character's race could be swapped. The casting aligns with the characters' depicted ethnicity within the film.