Guardians of the Galaxy Vol. 2 does not include any identifiable LGBTQ+ characters or themes. The narrative focuses on other aspects of character development and plot, with no explicit or implicit representation of queer identities or relationships.

Guardians of the Galaxy Vol. 2 does not feature any identifiable transsexual characters or themes. The narrative and character arcs do not touch upon trans identity, resulting in a rating of N/A for its portrayal.

The film features skilled female fighters like Gamora and Nebula. Gamora's significant close-quarters combat is primarily against another female character or non-human entities. Nebula's physical combat victories against male opponents rely heavily on her cybernetic enhancements, which are considered overwhelming technological superiority and thus excluded from the criteria.

All major and supporting characters in Guardians of the Galaxy Vol. 2 maintain the same gender as established in their Marvel Comics source material. No canonical male characters were portrayed as female, nor vice-versa.

Guardians of the Galaxy Vol. 2 features characters who are primarily aliens, and their on-screen portrayals are consistent with their established comic book species and appearances. No character canonically established as one human race in source material is portrayed as a different human race in the film.