The film 'John Wick' does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on John Wick's personal vendetta and the criminal underworld, without incorporating queer identities or storylines.

John Wick does not feature any identifiable transsexual characters or themes. The narrative focuses on a retired hitman's quest for revenge, with no elements pertaining to transgender identity or experiences.

The film features Ms. Perkins, a female assassin, who engages in close-quarters physical combat with John Wick. However, she is ultimately defeated by him in this encounter. No female character is depicted as victorious in direct physical combat against one or more male opponents.

John Wick (2014) is an original film that introduces new characters. There are no pre-existing characters from prior canon or historical figures whose gender was changed for this movie.

John Wick (2014) is an original film that introduced its characters for the first time. There is no prior source material, historical record, or previous installment from which characters' races were established and subsequently changed.