Knight and Day does not feature any identifiable LGBTQ+ characters, relationships, or themes. Consequently, there is no portrayal to evaluate within the scope of this framework.

Knight and Day does not feature any identifiable transsexual characters or themes within its narrative. Consequently, the film offers no portrayal, positive or negative, of transsexual individuals or experiences.

The film primarily features June Havens as the main female character, who is largely a civilian caught in a spy plot. While she is present in action sequences, her role does not include direct, close-quarters physical combat victories against male opponents. Her contributions are often accidental or involve the use of firearms in a broader shootout context, which falls outside the specified criteria.

Knight and Day is an original film with no pre-existing source material, historical figures, or legacy characters. All characters were created specifically for this movie, thus precluding any instances of gender swaps.

Knight and Day is an original film with no pre-existing source material or historical figures. All characters were created for this movie, thus there is no prior canonical race to be altered.