Muppets Most Wanted does not include any identifiable LGBTQ+ characters or themes. The film's narrative focuses on the Muppets' world tour and a case of mistaken identity, without any elements related to LGBTQ+ representation.

The film "Muppets Most Wanted" does not feature any identifiable transsexual characters or themes. The narrative focuses on the Muppets' European tour and a criminal plot, with no elements related to transgender identity or experiences.

The film features the established Muppet characters who retain their canonical genders. The primary human characters are new to this installment and do not represent gender-swapped versions of previously established characters from the franchise or other source material.

The film primarily features puppet characters, to whom the concept of human race does not apply. All significant human characters introduced in the film are new and original to this installment, not established legacy characters whose race was changed.