Night Is Short, Walk On Girl does not feature any identifiable transsexual characters or themes. The narrative explores themes of youth, love, and the absurdities of life through the whimsical adventures of its main protagonists and a host of eccentric supporting characters, none of whom are depicted as transsexual.

The movie does not contain any action or adventure elements.

The film is an adaptation of a novel by Tomihiko Morimi. All major characters, including the unnamed male protagonist "Senpai" and female protagonist "The Girl," retain their established genders from the source material in the film adaptation.

The film is a Japanese animated adaptation of a Japanese novel, featuring characters depicted as ethnically Japanese. There are no instances where a character established as one race in the source material is portrayed as a different race in the film.

Not depicted in the film.