The animated film 'Niko 2: Little Brother, Big Trouble' does not include any discernible LGBTQ+ characters or themes. Its narrative centers on a young reindeer's family life and challenges, with no elements related to queer identity present in the story.

The film "Niko 2: Little Brother, Big Trouble" does not feature any identifiable transsexual characters or themes. The narrative focuses on the adventures of a young reindeer and his family, with no elements related to transgender identity present in the story.

The film features female characters such as Niko's mother, Oona, and the antagonist, White Wolf. While White Wolf leads a pack of male wolves, she is ultimately defeated by the protagonists. No female character is depicted winning direct physical combat against one or more male opponents through skill or strength.

The film is a sequel that introduces new characters, such as Niko's stepbrother Jonni and the antagonist White Wolf's sister. However, no established character from the previous installment or source material has their canonical gender altered in this film.

The film features animated animal characters, for whom the concept of human racial categories and thus a 'race swap' does not apply. No characters were established as one race and then portrayed as another.