The film features gender-nonconforming characters who are portrayed as capable, fiercely loyal allies with significant agency. While the depiction incorporates classic comedic tropes associated with queer coding in anime, these elements are secondary to the characters' strength, bravery, and unwavering support for the protagonist, resulting in an incidental and overall balanced representation.

The film features gender-nonconforming and queer-coded characters, such as those from the Kamabakka Kingdom, whose identities are presented in line with the established lore of the broader franchise. These depictions maintain a mix of eccentric comedic tropes and genuine loyalty, resulting in an incidental portrayal that neither deeply affirms nor negatively disparages transgender themes.

The television special features Boa Hancock engaging in direct physical combat against male opponents. She successfully defeats multiple male adversaries using her martial arts and physical agility during the confrontations.

The narrative centers on a 'chosen family' dynamic where a surrogate brother figure and a dedicated crew bond through mutual loyalty and shared trauma following a major loss. While it champions deep personal bonds of loyalty and mentorship, it operates entirely outside the traditional nuclear family structure.