This film does not feature any identifiable transsexual characters or themes. The narrative focuses on the comedic spy adventures of OSS 117, with no elements related to transgender identity or experiences present in the storyline.

The film features Zéphyrine Bamba, a capable female agent who participates in action sequences. However, her contributions to combat are primarily through strategic actions, intelligence gathering, or the use of firearms. There are no clear instances where she defeats one or more male opponents in direct physical combat.

The film is the third installment in a comedic spy series featuring the character OSS 117, who originated in novels. The main character, Hubert Bonisseur de La Bath (OSS 117), remains consistently male, portrayed by Jean Dujardin, as in previous iterations. No other established characters from prior canon or real-world history have their gender changed in this film.

The film features the established character OSS 117, consistently portrayed by a white actor. New characters are introduced, but no existing character with a previously established race is depicted by an actor of a different race.