Pooh's Grand Adventure: The Search for Christopher Robin does not include any discernible LGBTQ+ characters or themes. The narrative focuses on the established friendships and adventures of the Hundred Acre Wood inhabitants, without exploring queer identities or relationships.

This animated film, part of the Winnie the Pooh franchise, centers on the adventures of Pooh and his friends in the Hundred Acre Wood. It does not feature any identifiable transsexual characters or themes, nor does it touch upon gender identity in any capacity.

The film features Kanga as the primary female character, who is portrayed as a nurturing figure. There are no scenes depicting Kanga or any other female character engaging in or winning physical combat against male opponents.

All established characters from the Winnie-the-Pooh canon, including Pooh, Piglet, Tigger, Rabbit, Eeyore, Owl, Kanga, Roo, Christopher Robin, and Gopher, retain their original genders as depicted in source material and prior adaptations.

The film features anthropomorphic animal characters and Christopher Robin, who is consistently depicted as a white boy, aligning with his established portrayal in A.A. Milne's original works and prior animated adaptations. No character's race was altered from their canonical or historical depiction.