The film does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the established adventures of Sherlock Holmes and Dr. Watson without explicitly exploring queer identities or relationships.

Sherlock Holmes: A Game of Shadows does not include any identifiable transsexual characters or explore related themes. The narrative focuses on the conflict between Sherlock Holmes and Professor Moriarty, with no elements pertaining to transgender identity.

The film features female characters such as Irene Adler and Madam Simza Heron. However, neither character engages in or is depicted as victorious in close-quarters physical combat against one or more male opponents. Their roles do not involve direct physical combat feats.

The film features established characters from Arthur Conan Doyle's Sherlock Holmes canon, such as Holmes, Watson, Moriarty, and Mycroft. All these characters are portrayed on screen with the same gender as their original canonical depiction. New characters introduced in the film do not constitute a gender swap.

All major and legacy characters in Sherlock Holmes: A Game of Shadows, including Sherlock Holmes, Dr. Watson, and Professor Moriarty, are portrayed by actors whose race aligns with their established canonical depictions. No instances of race swapping were identified.