The film 'Teenage Mutant Ninja Turtles' does not feature any identifiable LGBTQ+ characters or themes. Its narrative is centered on the titular heroes, their allies, and their conflict with the Foot Clan, resulting in no portrayal of LGBTQ+ elements.

The film 'Teenage Mutant Ninja Turtles' (1990) does not feature any identifiable transsexual characters or themes. Its narrative centers on martial arts, crime-fighting, and friendship, with no elements related to transgender identity or experiences.

The film features April O'Neil as the primary female character. While she is present during many action sequences and is a target of the Foot Clan, she does not engage in or win any direct physical combat against male opponents. No other female characters participate in combat.

The 1990 film adaptation of Teenage Mutant Ninja Turtles retains the established genders for all its core characters, including the four turtles, Splinter, April O'Neil, Casey Jones, and Shredder, consistent with their comic book origins.

The 1990 film adaptation accurately portrays the established races of its human characters, April O'Neil and Casey Jones, as white, consistent with their comic book origins. The anthropomorphic turtles and Splinter do not have a human race.