The film 'The Girl Who Kicked the Hornet's Nest' does not feature any identifiable transsexual characters or themes. Its plot is centered on political intrigue, legal proceedings, and the protagonists' fight against corruption, without addressing gender identity issues.

The film focuses on Lisbeth Salander's recovery from injuries and subsequent legal battle. While she confronts male antagonists, her victories are achieved through strategic planning, manipulation, or the use of external forces rather than direct physical combat.

This film is a direct adaptation of the third novel in Stieg Larsson's Millennium series. All major and minor characters maintain the same gender as established in the original source material and prior film adaptations.

This film is an adaptation of a Swedish novel and features characters consistently portrayed by actors whose race aligns with their established depictions in the source material and prior installments. No character's race was altered from their original canon.