The League of Extraordinary Gentlemen does not include any explicit or implicitly identifiable LGBTQ+ characters or themes. The narrative focuses on a diverse group of literary characters engaged in a Victorian-era adventure without addressing queer identity or experiences.

The film "The League of Extraordinary Gentlemen" does not feature any identifiable transsexual characters or themes. Its narrative focuses on a team of literary figures with various supernatural abilities and does not touch upon gender identity or transition in any capacity.

The film's main characters, including Allan Quatermain, Captain Nemo, Mina Harker, Dr. Jekyll/Mr. Hyde, Dorian Gray, and Tom Sawyer, retain their established genders from their respective literary sources. The Invisible Man character, Rodney Skinner, is a new character for the film, and his gender is consistent with the canonical Invisible Man (Griffin).

All major characters in the film, including Allan Quatermain, Captain Nemo, Mina Harker, and Dr. Jekyll, are portrayed by actors whose race aligns with their established canonical or historical depictions. There are no instances of a character's race being changed from their source material.