The film does not feature any identifiable transsexual characters or themes. The narrative focuses on a detective mystery involving espionage, disguises, and mistaken identities, none of which are related to gender identity or transsexuality.

The film features Gabrielle Valladon as the primary female character. Her role involves espionage and mystery, but she is not depicted engaging in or winning close-quarters physical combat against any male opponents. No female characters participate in such action scenes.

The film portrays established characters like Sherlock Holmes and Dr. Watson with their canonical male genders. No characters from the source material or historical figures are depicted with a different gender.

The film features Sherlock Holmes, Dr. Watson, and other characters consistent with their established racial depictions in Arthur Conan Doyle's original stories and prior adaptations. No characters canonically, historically, or widely established as one race are portrayed as a different race.