The Tale of Despereaux does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on a mouse's bravery and a rat's quest for revenge, without incorporating queer identities or experiences into its fantastical world.

The animated film "The Tale of Despereaux" does not feature any identifiable transsexual characters or themes. The narrative focuses on a mouse, a rat, a princess, and a serving girl in a fantasy kingdom, with no elements related to transgender identity or experiences.

The film does not feature any female characters engaging in or winning direct physical combat against one or more male opponents. Princess Pea is a damsel in distress, and Miggery Sow, while involved in the plot, does not achieve combat victories.

The film adaptation of "The Tale of Despereaux" faithfully maintains the genders of all established characters from the original novel. No characters canonically, historically, or widely established as one gender are portrayed as a different gender in the movie.

The film adapts a fantasy novel featuring anthropomorphic animals and human characters. The human characters' on-screen portrayals align with the implicit racial context of the source material, which does not specify race but is set in a European-inspired world. No characters established as one race in the book are depicted as a different race in the movie.