Transformers: Revenge of the Fallen does not include any discernible LGBTQ+ characters or themes. The narrative focuses on action, military conflicts, and heterosexual relationships, with no representation of queer identities or experiences, resulting in a 'N/A' rating for LGBTQ+ portrayal.

Transformers: Revenge of the Fallen does not include any identifiable transsexual characters or themes. The narrative focuses on the conflict between Autobots and Decepticons, with human characters primarily serving to advance the robot-centric plot, without exploring gender identity.

The film features female characters like Mikaela Banes and the Autobot Arcee, who participate in action sequences. However, their combat roles do not include direct physical victories against male opponents using hand-to-hand or melee skills. Alice, a Decepticon, engages in physical combat with Sam but is ultimately defeated.

The film features established Transformers characters who retain their canonical genders from previous iterations and source material. New characters introduced do not have pre-existing gender identities that are then swapped.

The film primarily features human characters whose races align with their established portrayals or are new creations. The robot characters, being alien machines, do not possess a human-defined race, thus precluding a race swap.