Due to the absence of any provided film content or plot details for 'Trouble', an evaluation of its portrayal of LGBTQ+ characters and themes could not be conducted. Therefore, the net impact is rated as N/A.

Without access to the specific content of the film 'Trouble,' it is not possible to identify or analyze scenes involving female characters in physical combat against male opponents. Therefore, a determination of combat feats cannot be made.

The film "Trouble" (2019) is an original animated story. It is not an adaptation of existing material, a biopic, or a reboot of a series with established characters. Therefore, no characters exist from prior canon or history whose gender could have been swapped.

The film "Trouble" (2019) is an original animated production about a dog, not an adaptation of existing material with pre-established character races. Therefore, no characters were canonically, historically, or widely established as one race prior to this film.