The film 'Two by Two: Overboard!' does not feature any identifiable LGBTQ+ characters or themes. The narrative focuses on the adventures of animal characters on Noah's Ark, without addressing queer identity in any capacity, resulting in no depiction.

The animated film "Two by Two: Overboard!" does not feature any identifiable transsexual characters or themes. The narrative focuses on animal characters and their adventure during a flood, with no elements related to transgender identity or experiences.

The film is an animated children's adventure. While female characters like Leah are resourceful and brave, their challenges are primarily environmental or involve large, non-gendered creatures. There are no scenes depicting a female character defeating one or more male opponents in direct physical combat.

This animated film is a direct sequel, continuing the story with the same established characters from the previous installment. All returning characters maintain their original genders, and new characters introduced do not have prior canonical genders to be swapped from.

This animated film features original animal characters. The concept of a 'race swap,' as defined for human or human-like characters with established racial identities, does not apply to this movie.