Scooby-Doo! Stage Fright does not include any explicit or implicit LGBTQ+ characters or themes. The narrative focuses on the mystery of a phantom and a talent show, without incorporating elements related to queer identity or experiences.

The film "Scooby-Doo! Stage Fright" does not feature any identifiable transsexual characters or themes. The story centers on the Scooby gang solving a mystery at a haunted opera house, with no elements related to transgender identity or experiences.

The film features female characters like Daphne Blake and Velma Dinkley, who participate in the mystery-solving and chase sequences. However, there are no scenes where a female character is depicted as victorious in direct physical combat against one or more male opponents.

The film features the established Scooby-Doo characters—Scooby, Shaggy, Fred, Daphne, and Velma—all retaining their canonical genders. New characters introduced for this specific story do not constitute gender swaps.

The film features the established Scooby-Doo characters (Fred, Daphne, Velma, Shaggy) who are consistently portrayed as white, aligning with their long-standing canonical depictions. No characters established as one race in prior canon are depicted as a different race in this installment.