This Scooby-Doo animated film does not feature any discernible LGBTQ+ characters or themes. The plot centers on the gang's space adventure and monster mystery, with no elements related to queer identity present in the narrative.

The film "Scooby-Doo! Moon Monster Madness" does not feature any identifiable transsexual characters or themes. The narrative focuses on a space adventure with the Scooby-Doo gang, and there are no elements within the plot or character arcs that pertain to transsexual identity or experiences.

The film primarily features the Scooby-Doo gang solving a mystery involving 'moon monsters' in space. The female characters, Daphne and Velma, contribute through investigation and setting traps, but do not engage in or win direct physical combat against male opponents. Victories are achieved through unmasking and outsmarting villains.

The film features the classic Scooby-Doo gang—Fred, Daphne, Velma, Shaggy, and Scooby—all portrayed with their long-established canonical genders. No legacy characters from the franchise have their gender altered in this installment.

The film features the established Scooby-Doo characters (Fred, Daphne, Velma, Shaggy) who are consistently portrayed as white, matching their long-standing canonical depictions. No character originally established as one race is depicted as a different race in this installment.