No LGBTQ+ characters or themes appear in the special. The narrative focuses on bravery, friendship, and environmental harmony among animal characters in a jungle adventure.

No transgender characters or themes appear in the film. The story centers on a jungle adventure without any related elements.

Female characters like Skye and Lucita engage in rescue operations and vehicular pursuits but do not participate in close-quarters physical combat against male opponents. Actions involve gadgets, vehicles, and extractions rather than hand-to-hand fights.

Valiente: A Tracker Story uses established PAW Patrol characters such as Tracker, Ryder, and the pups, all portrayed with their canonical genders. New characters like Lucita Mala are original additions without gender alterations from prior material.

Valiente: A Tracker Story continues the PAW Patrol franchise with established characters maintaining their canonical racial depictions in animation, and new characters introduced without reference to prior source material that would enable race swaps.