WALL·E does not feature any identifiable LGBTQ+ characters or themes. The central romantic relationship is between the male-coded robot WALL·E and the female-coded robot EVE. The film's narrative focuses on environmentalism, consumerism, and the nature of love and humanity, without incorporating LGBTQ+ specific elements.

WALL·E does not feature any identifiable transsexual characters or themes. The narrative focuses on robots and a future human society, without exploring gender identity or related topics.

The film features EVE, a female robot, who engages in action sequences primarily using her integrated plasma cannon. While she physically resists the male-coded autopilot AUTO during a critical struggle, her actions are more about achieving an objective and resisting, rather than directly defeating AUTO in close-quarters physical combat. The final disabling of AUTO is performed by Captain McCrea.

WALL·E is an original film with no pre-existing source material or legacy characters. All characters were created for this specific production, thus no gender swaps occurred.

WALL·E is an original animated film featuring robots and human characters created specifically for this movie. There are no pre-existing characters from source material or history whose race could have been altered.