Zathura: A Space Adventure does not feature any identifiable LGBTQ+ characters or themes. The narrative centers on a family's adventure through space, with no elements pertaining to queer identity or experiences.

Zathura: A Space Adventure does not feature any identifiable transsexual characters or themes. The narrative focuses on a space-themed board game and the adventures of two brothers and their sister, with no elements related to transgender identity.

The film features Lisa, the older sister, as the primary female character. While she is present during various perilous situations, she does not engage in or win any direct physical combat against male opponents. Her actions are primarily focused on survival and interacting with the game's challenges.

The film adapts Chris Van Allsburg's book "Zathura." All primary characters from the source material, including Walter, Danny, Lisa, and the Astronaut, retain their original genders in the film adaptation. No established character undergoes a gender change.

The film's main characters, Walter, Danny, and Lisa Budwing, are portrayed by actors whose race aligns with their depiction in the original book. The Astronaut character is an original creation for the film, not a race-swapped character from the source material.