All in the Family frequently depicted LGBTQ+ characters and themes, primarily through Archie Bunker's prejudiced reactions. However, the narrative consistently challenged Archie's bigotry, portraying LGBTQ+ characters with dignity and using other characters to advocate for acceptance, thereby critiquing homophobia rather than endorsing it.

The show features Beverly LaSalle, a transsexual woman, depicted with dignity and talent. While Archie Bunker expresses transphobic views, these are consistently challenged and condemned by other characters, particularly Edith. The narrative uses Archie's bigotry to critique prejudice, ultimately affirming the worth of trans lives through empathetic portrayal and character responses.

The show critiques the hypocrisy and bigotry often cloaked in Christian rhetoric (Archie's views) but simultaneously portrays genuine, compassionate Christian faith (Edith's character) as a moral good, positioning the audience to condemn prejudice.

The show consistently uses Archie's anti-Semitic remarks to satirize and condemn prejudice, positioning the audience to sympathize with Jewish characters and view bigotry as ignorant and wrong.