All in the Family featured groundbreaking LGBTQ+ portrayals, including a gay friend and the recurring drag queen Beverly LaSalle. While Archie Bunker initially reacted with prejudice, the show consistently critiqued his bigotry. Through character arcs like Archie's eventual empathy for Beverly, the series humanized LGBTQ+ individuals and challenged societal prejudice, affirming their worth despite tragic elements.

The show features Beverly LaSalle, a transsexual woman, depicted with dignity and talent. While Archie Bunker expresses transphobic views, these are consistently challenged and condemned by other characters, particularly Edith. The narrative uses Archie's bigotry to critique prejudice, ultimately affirming the worth of trans lives through empathetic portrayal and character responses.

The show critiques the hypocrisy and bigotry often cloaked in Christian rhetoric (Archie's views) but simultaneously portrays genuine, compassionate Christian faith (Edith's character) as a moral good, positioning the audience to condemn prejudice.

The show consistently uses Archie's anti-Semitic remarks to satirize and condemn prejudice, positioning the audience to sympathize with Jewish characters and view bigotry as ignorant and wrong.