Batman: The Animated Series, produced in the 1990s, does not explicitly feature LGBTQ+ characters or themes. Its narrative focuses on established Batman lore and character development without incorporating overt queer representation, which was uncommon for mainstream children's animation at the time.

Batman: The Animated Series does not feature any identifiable transsexual characters or themes within its narrative. Consequently, there is no portrayal to evaluate against the provided rubric for positive, negative, or neutral impact.

Batman: The Animated Series faithfully adapted the established genders of its core cast and villains from the DC Comics source material. No character canonically established as one gender was portrayed as a different gender in the show. New characters introduced do not count as gender swaps.

Batman: The Animated Series faithfully adapted the established racial depictions of its core characters from the DC Comics source material. No characters canonically established as one race were portrayed as a different race in the series.