Bob Hearts Abishola features LGBTQ+ characters, notably Christina, whose bisexual identity and relationship are explored with sensitivity and family acceptance, and Kofo, a gay character whose sexuality is normalized. The show depicts these characters with dignity and complexity, affirming their identities without relying on harmful stereotypes or making their sexuality a source of ridicule.

The show portrays Nigerian Christianity as a foundational element of Abishola's family and community, emphasizing its role in providing moral guidance, cultural identity, and strong social bonds. While cultural differences and strict interpretations are sometimes sources of humor, the narrative consistently treats the faith with respect and highlights its positive influence.