Bobobo-bo Bo-bobo is a surreal comedy anime known for its absurd humor, which includes frequent instances of gender-bending and cross-dressing for comedic effect. However, these elements are consistently presented as temporary gags or visual jokes, rather than exploring themes of gender identity or featuring characters who identify as transsexual. Consequently, the series does not contain identifiable transsexual characters or themes as defined by the rubric.

In Bobobo-bo Bo-bobo, female characters such as Rem, Ruby, and Suzy engage in combat against male opponents, primarily the protagonists. However, these female characters are consistently defeated in their physical confrontations, often utilizing unique hair-based abilities or other cartoon powers. There are no clear instances where a female character achieves victory over one or more male opponents through skill, strength, or martial arts.

The anime adaptation of "Bobobo-bo Bo-bobo" maintains the established genders of its characters from the original manga. While the show features comedic gender-bending disguises and transformations, these are temporary in-plot gags and do not constitute a permanent re-gendering of any canonically established character.

Bobobo-bo Bo-bobo is a Japanese anime adaptation of a Japanese manga. The characters are consistently portrayed in a manner typical of Japanese animation, reflecting their original East Asian ethnicity. There are no instances of characters established as one race in the source material being depicted as a different race in the anime.