No transgender characters or themes appear in the film. The narrative follows the backstory of key sorcerers in a world of curses and battles, focusing on friendship and duty among characters like Gojo and Geto, with no elements addressing trans identity.

Female characters like Riko Amanai and Misato Kuroi appear but do not engage in victorious physical combat against male opponents. Misato briefly scuffles with a curse user who escapes. Other females such as Mei Mei and Utahime Iori support missions without direct fights. All major combats involve male sorcerers using cursed techniques.

The film adapts the Hidden Inventory and Premature Death arcs faithfully, retaining the canonical genders of all major characters such as Satoru Gojo, Suguru Geto, and Riko Amanai without any swaps.

All major characters from the Jujutsu Kaisen manga's Hidden Inventory arc are Japanese and are portrayed as such by Japanese voice actors in the animated film, resulting in no race swaps.