Cold Case frequently features LGBTQ+ characters, often as victims of crimes motivated by prejudice. While outcomes are tragic, the show's narrative consistently condemns homophobia and transphobia, portraying LGBTQ+ individuals with dignity and empathy, and affirming the value of their lives and relationships.

Cold Case features multiple episodes centered on the murders of transsexual women. The series consistently portrays these characters with dignity and empathy, highlighting their struggles for identity and acceptance. While their stories often end tragically due to external prejudice and violence, the narrative affirms the worth of their lives and condemns the discrimination they faced.

The series frequently portrays Christian characters and communities, exploring their moral complexities and the role of faith in their lives. While individual adherents or institutions may be depicted with flaws or involved in crimes, the narrative consistently critiques human failings rather than the core tenets of the faith, often highlighting faith as a source of comfort, community, or moral guidance.

When Judaism is depicted, particularly in the context of historical crimes or prejudice, the narrative consistently condemns antisemitism and bigotry. The show positions the audience to sympathize with Jewish characters or communities who are victims of such prejudice, aligning the narrative with the dignity of the faith.