CSI: Vegas features multiple LGBTQ+ characters, including bisexual Allie Rajan and gay Bryan Roby. Their identities are portrayed with dignity, complexity, and agency, integrated naturally into the narrative. Relationships and personal journeys are depicted supportively, affirming the worth of LGBTQ+ lives and love without relying on harmful stereotypes or making identity a source of misery.

CSI: Vegas features a trans woman character, Serena, as a crime victim in one episode. The show portrays her with dignity, and the investigation treats her identity respectfully. The narrative avoids harmful stereotypes, focusing on her personhood and the crime, thereby affirming the worth of trans lives despite the tragic circumstances.

The series frequently depicts Christian institutions or individuals within Christian communities as being involved in or covering up crimes, exposing hypocrisy, fanaticism, or moral failings. While individual characters may hold genuine faith, the narrative often uses religious contexts to highlight human corruption and the darker aspects of society, without consistently affirming the faith's virtues.