Glitch Techs features multiple LGBTQ+ characters, including a bisexual protagonist, a lesbian recurring character, and a non-binary character. Their identities are presented respectfully and are integrated into the show's diverse cast without being a source of conflict, mockery, or negative stereotypes, contributing to an affirming portrayal.

Glitch Techs features Zahra, a character confirmed by creators as a trans girl. Her identity is not explicitly addressed in the show; she is simply presented as a normal student, neither uplifted nor denigrated for her trans identity. The portrayal is incidental, avoiding strong positive or negative narrative arcs.