Highlander: The Series features a transsexual Immortal character, Michelle Webster, in the episode 'Chivalry.' The show portrays her with dignity, framing the antagonist's transphobia as villainous. The protagonist's eventual acceptance and defense of Michelle result in a net positive and affirming portrayal of her identity.

The show features several female Immortal characters, such as Amanda Darieux, who are skilled sword fighters. These characters frequently engage in and win close-quarters physical combat against male Immortal opponents.

The series portrays Buddhist philosophy and practices, particularly through characters like Kage, as a source of profound wisdom, inner peace, and ethical guidance, offering a positive contrast to the violence of the Immortal world.

The series frequently depicts Christian institutions, particularly the historical Church, as dogmatic, persecutory, and a source of conflict for Immortals, often leading to violence or misunderstanding. While individual believers may be shown positively, the overall narrative critiques the oppressive aspects of organized Christianity.