Lilo & Stitch: The Series does not feature any identifiable LGBTQ+ characters or themes. The narrative primarily focuses on themes of 'ohana' (family), acceptance of differences, and adventure, without engaging with queer identity or experiences.

Lilo & Stitch: The Series does not feature any identifiable transsexual characters or themes. The narrative focuses on Lilo, Stitch, and other alien experiments, with no elements related to transgender identity present in the series.

The series primarily features Lilo and Nani as main female characters. While Nani is physically capable, her direct confrontations with male antagonists like Gantu typically result in her being outmatched or relying on Stitch for assistance, rather than her achieving victory through skilled physical combat. Lilo's successes are generally due to her ingenuity or the unique abilities of the experiments. No female character is depicted defeating male opponents in close-quarters physical combat through skill or strength.

The series is a direct continuation of the 2002 film, and all established characters from the original movie retain their canonical genders. New characters introduced in the series do not qualify as gender swaps.

The animated series continues the story of the original film, maintaining the established racial depictions of its characters. No characters who were canonically or visually established as one race in the source material are portrayed as a different race.